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CD15鼠單克隆抗體

CD15鼠單克隆抗體

  • 價格￥600
  • 規(guī)格100ul
  • 貨號HKZ150263
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150263CD15鼠單克隆抗體100ul支600
CD15
Mouse?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50140?Clone# ?MMA
Predicted?Molecular Wt:
59kDa
Purity:??Affinity?purified?IgM
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P22083
Background:
CD15?is?present on greater than 90% of granulocytes?including?neutrophils?and?eosinophils,?and?to?a?lesser?degree,?on?monocytes.?CD15?is expressed?in?Reed-Sternberg cells of?Hodgkin’s disease?(of the?nodular?sclerosis,?mixed?cellularity?and?lymphocyte-
depleted subtypes), and certain types of?epithelial?cells.?It?is?generally?agreed?that?the?Reed-Sternberg?cell?variants?in?lymphocyte-?predominant?Hodgkin’s disease are?not?reactive with CD15.?Positive staining?for?CD15?combined?with?a?negative?reaction?for
lymphocytic?markers?may?provide support for?Hodgkin’sdisease.
Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer
(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)
for?30?minutes.
Immunogen:
Immunized?with?the?U-937?histiocytic?cell?line
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA
0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store
at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Pellegrini?W, et?al.?Haematologica.?2007?May;
92(5):708-9.
2.?Song?JY, et?al. Am?J?Surg?Pathol.?2011?May;?35(5):767-?72.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
Hodgkin’s?Lymphoma
tissue?labelling?CD15 with
MMA.?Heat?mediated
antigen?retrieval was
performed?using Tris/EDTA
buffer?pH?9.0

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