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Desmin兔單克隆抗體

Desmin兔單克隆抗體

  • 價(jià)格￥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150262
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150262Desmin兔單克隆抗體100ul支600
Desmin
Mouse?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50139?Clone# ?D33
Predicted?Molecular Wt:
53kDa
Purity: ??ProG?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P17661
Background:
Desminis a?53kDa?intermediate filament?(IF)?protein that?integrates the sarcolemma, Z?disk,?and?nuclear?membrane?in
sarcomeres and?regulates sarcomere architecture.?Desmin?play?no?role?in contractility?but?serves to?maintain the?orientation?of?actin and?myosin filaments and?may also?play?a?role?in?nuclear transcription.
Desminis expressed?in all striated?muscle cells?and?most?smooth?muscle?cells.?In the?developing?striated?muscle?cells?it?replaces?vimentin,?linked to the Z-disc.?In the smooth?muscle cells desminis?associated?with?cytoplasmic?dense?bodies?and
subplasmalemmal dense?plaques.?In?myofibroblasts and vascular smooth?muscle cells?desminis?co-expressed?with?vimentin.
However,?in the arterial smooth?muscle cells, desmin?may?be?scarce?or?absent,?while?vimentin?expression?is?retained.?Mesothelial?cells?may express desmin,?particularly?in effusions and?cultures.?In?tumor?tissues,?it?detected?in?leiomyomas,?leiomyosarcoma,
rhabdomyomas,?rhabdomyosarcoma, and?perivascular cells of glomus tumors of the?skin.
Desminis?used?in a?panel for?identification of?leiomyosarcoma,?rhabdomyosarcoma and?other tumours with?myoid?differentiation, and for classification of?mesenchymal,?pleomorphic,?spindle cell?and?round?cell?neoplasms.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Immunogen derived from?human?Leiomyoma
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Brodehl?A., et?al.?Circ?Cardiovasc?Genet.?2013?Dec;6(6):615-23.
2.?Colella?R., et?al. Am?J?Surg?Pathol.?2010 Jan;34(1):10-7.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
esophagus tissue?labelling?Desmin?with?D33.?Heat
mediated?antigen?retrieval?was?performed?using
Tris/EDTA?buffer?pH?9.0

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