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ERG兔單克隆抗體

ERG兔單克隆抗體

  • 價格¥1000
  • 規(guī)格100ul
  • 貨號HKZ150246
  • 單位
  • 品牌HaoKebio

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品牌商品編碼商品名稱規(guī)格單位單價
HaoKebioHKZ150246ERG兔單克隆抗體100ul1000
ERG
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50123?Clone# ?BP6127
Predicted?Molecular Wt:
55kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P11308
Background:
ERG?(ETS-related gene)?is a?proto-oncogene, a?member of the?ETS family?of transcription?factors.?The?ERG?gene?encodes?for?a?nuclear?protein, also called?ERG, which?is?involved?in?hematopoietic and?endothelial?development.?ERG?remains?constitually????expressed?in?endothelial cells?in?blood and?lymphatic vessels, and?in?bone?marrow stem?cells.
ERG?is expressed?in virtually allendothelial?neoplasms?including?haemangioendothelioma, angiosarcoma and?Kaposi?sarcoma.?ERG?is overexpressed secondary to gene?rearrangement?in cases?of?prostate?adenocarcinoma,?gastrointestinal?stromal
tumour, synovial sarcoma,?meningioma, epithelioid sarcoma,?malignant?rhabdoid tumour,?acute?myeloid?leukemia?and?blastic?extramedullary?myeloid tumor, and?rarely?Ewing sarcoma /?primitive?peripheral?neuroectodemal tumour, chondrosarcoma,
osteosarcoma, and?rhabdomyosarcoma.
For the?identification of endothelial differentiation?ERG seems?more?sensitive?and?specific?than?any?other?marker.?Moreover,?????the?interpretation?is often easier due to the?nuclear?reaction, which?also?allows?for?double?stains?with?cytoplasmic?markers?like?podoplanin. Among carcinomas,?ERG?is?highly specific for?prostate, while the?sensitivity?is?moderate.
Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within
aa450 to?C-terminus?of?ERG?was?used?as?an immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Miettinen?M.?Histopathology.?2014 Jan;64(1):101-18.
2.?Stockman?DL,?Mod?Pathol.?2014 Apr;27(4):496-501.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
appendix?labelling?ERG?with?BP6127.?Heat
mediated?antigen
retrieval was?performed?using Tris/EDTA?buffer????pH?9.0.

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